gastric cancer cell line ags  (ATCC)

Journal: iScience

Article Title: KLF13-mediated CES2 upregulation via p300-dependent acetylation sensitizes gastric cancer cells to irinotecan

doi: 10.1016/j.isci.2025.114199

Figure Lengend Snippet: KLF13 expression correlates with CES2 and CPT-11 sensitivity in GC (A) Heatmap depicting the relative mRNA expression of KLF13 and CES2 in 37 pairs of GC tissues and adjacent normal tissues. The Pearson correlation coefficient (r) and its significance ( p value) are shown on the left (r = 0.58, p < 0.001). (B) Baseline characterization of NUGC4 and AGS GC cell lines. (Left) western blot (WB) analysis of endogenous KLF13 and CES2 proteins. (Middle) qPCR analysis of KLF13 and CES2 mRNA. Expression in AGS cells is relative to NUGC4 (set to 1). (Right) Cell viability curves after 48 h CPT-11 treatment. (C) Correlation between KLF13/CES2 mRNA expression and ORR in 30 GC patients receiving CPT-11-based second-line chemotherapy. Patients were stratified into high/low groups by the median. ORR was compared using Fisher’s exact test. Data in (B) are mean ± SD ( n = 3). ∗ p < 0.05.

Article Snippet: The human gastric cancer cell lines NUGC4 (JCRB0834) and AGS (ATCC CRL-1739) were obtained from the Japanese Collection of Research Bioresources (JCRB) and the American Type Culture Collection (ATCC), respectively.

Techniques: Expressing, Western Blot

Journal: iScience

Article Title: KLF13-mediated CES2 upregulation via p300-dependent acetylation sensitizes gastric cancer cells to irinotecan

doi: 10.1016/j.isci.2025.114199

Figure Lengend Snippet: KLF13 directly regulates CES2 transcription (A and B) WB (left) and qPCR (right) analysis of CES2 expression in NUGC4 and AGS cells 48 h after (A) KLF13 overexpression (OE) or (B) KLF13 knockdown (si-KLF13). See also for KLF13 expression efficiency. (C) Luciferase activity of serially deleted CES2 promoter constructs co-transfected with KLF13 OE or Mock vector in NUGC4 and AGS cells. (D) Schematic of the CES2 promoter (−1,312 bp) showing predicted KLF13-binding sites from JASPAR. (E) Luciferase activity of the −1,312-bp promoter with individual site mutations. (F) Luciferase activity of the −1,312-bp promoter with all three sites mutated (Triple-Mut). Data are mean ± SD ( n = 3). ns, not significant. ∗ p < 0.05.

Article Snippet: The human gastric cancer cell lines NUGC4 (JCRB0834) and AGS (ATCC CRL-1739) were obtained from the Japanese Collection of Research Bioresources (JCRB) and the American Type Culture Collection (ATCC), respectively.

Techniques: Expressing, Over Expression, Knockdown, Luciferase, Activity Assay, Construct, Transfection, Plasmid Preparation, Binding Assay

Journal: iScience

Article Title: KLF13-mediated CES2 upregulation via p300-dependent acetylation sensitizes gastric cancer cells to irinotecan

doi: 10.1016/j.isci.2025.114199

Figure Lengend Snippet: KLF13 expression dictates cellular sensitivity to CPT-11 Cell viability curves of NUGC4 and AGS cells treated with CPT-11 for 48 h following (A) KLF13 overexpression or (B) KLF13 knockdown. Data are mean ± SD ( n = 3). ∗ p < 0.05 indicates significant difference between the two groups at the indicated concentration, determined by Student’s t test.

Article Snippet: The human gastric cancer cell lines NUGC4 (JCRB0834) and AGS (ATCC CRL-1739) were obtained from the Japanese Collection of Research Bioresources (JCRB) and the American Type Culture Collection (ATCC), respectively.

Techniques: Expressing, Over Expression, Knockdown, Concentration Assay